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Publications of the:

Bioinformatics group

Biosystems Technology group

Biocatalysis group

Fermentation and recovery group

All publicatios

Original papers in refereed journals:

in press:

Publications 2011:

[220] Sirim, D., 0, Wagner, F., Wang, L., Schmid, R.D., Pleiss, J., 2011. The Laccase Engineering Database: a classification and analysis system for laccases and related multicopper oxidases. Database bar006: - Medline

[219] Beuttler, H., 0, Hoffmann, J., Jeske, M., Hauer, B., Schmid, R., Altenbuchner, J., Urlacher, V., 2011. Biosynthesis of zeaxanthin in recombinant Pseudomonas putida. Appl.Microbiol.Biotechnol. 89: 11377-11347 Medline

Publications 2010:

[218] Liu, D., 0, Trodler, P., Eiben, S., Koschorreck, K., Müller, M., Pleiss, J., Maurer, S.C., Branneby, C., Schmid, R.D., 2010. Rational design of Pseudozyma antarctica lipase B yielding a general esterification catalyst. ChemBioChem 11: 789-795 Medline

[217] Liu, L., 0, Schmid, R.D., Urlacher, V.B., 2010. Engineering cytochrome P450 monooxygenase CYP 116B3 for high dealkylation activity.. Biotechnol Lett. : 0-0 Medline

[216] Liu D., 0, Trodler P., Eiben S., Koschorrek K., Mueller M., Pleiss J., Maurer S., Branneby C., Schmid R.D., 2010. Rational Design of Pseudozyma antarctica Lipase B Yielding a General Esterification Catalyst. Chem. Bio. Chem. 11: 789-795

[215] Koschorreck, K., 0, Liu, D., Kazenwadel, C., Schmid, R.D., Hauer, B., 2010. Heterologous expression, characterization and site-directed mutagenesis of cutinase CUTAB1 from Alternaria brassicicola. Appl. Microbiol. Biotechnol. 87: 991-997 Medline

[214] Roepcke, C.B., 0, Muench, S.B., Schulze, H., Bachmann, T.T., Schmid, R.D., Hauer, B., 2010. Analysis of phosphothionate pesticides using a chloroperoxidase pretreatment and acetylcholonesterase biosensor detection. J.Agric.Food Chem. 58: 8748-8756 Medline

Publications 2009:

[213] Trodler, P., 0, Schmid, R.D., Pleiss, J., 2009. Modeling of solvent-dependent conformational transitions in Burkholderia cepacia lipase. BMC Struct Biol 9: 38-0 Medline

[212] Dietrich, M., 0, Do, T.A., Schmid, R.D., Pleiss, J., Urlacher, V.B., 2009. Altering the regioselectivity of the subterminal fatty acid hydroxylase P450 BM-3 towards gamma- and delta-positions. J Biotechnol 139: 115-117 Medline

[211] Girhard, M., 0, Machida, K., Itoh, M., Schmid, R.D., Arisawa, A., Urlacher, V.B., 2009. Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system. Microb Cell Fact 8: 36-36 Medline

[210] Koschorreck, K., 0, Schmid, R.D., Urlacher, V.B., 2009. Improving the functional expression of a Bacillus licheniformis laccase by random and site-directed mutagenesis. BMC Biotechnol 9: 12-12 Medline

[209] Rosche B., 0, Li XZ., Hauer B., Schmid A., K. Bühler, 2009. Microbial biofilms: a concept for industrial catalysis? Trends Biotechnol.. 27(11): 636-43

Publications 2008:

[208] Trodler, P., 0, Nieveler, J., Rusnak, M., Schmid, R.D., Pleiss, J., 2008. Rational design of a new one-step purification strategy for Candida antarctica lipase B by ion-exchange chromatography. J Chromatogr A 1179: 161-167 Medline

[207] Messerschmidt, S.K.E, 0, Kolbe, A., Müller, D., Knoll, M., Pleiss, J, Kontermann, R.E., 2008. Novel single-chain Fv′ formats for the generation of immunoliposomes by site-directed coupling. Bioconjugate Chem. 19: 362-369 Medline

[206] Li, H.M., 0, Mei, L.H., Urlacher, V.B., Schmid, R.D., 2008. Cytochrome P450 BM-3 evolved by random and saturation mutagenesis as an effective indole-hydroxylating catalyst. Appl Biochem Biotechnol 1444: 27-36 Medline

[205] Koschorreck, K., 0, Richter, S.M., Swierczek, A., Beifuss, U., Schmid, R.D., Urlacher, V.B., 2008. Comparative characterization of four laccases from Trametes versicolor concerning phenolic C-C coupling and oxidation of PAHs. Arch Biochem Biophys 474: 213-219 Medline

[204] Koschorreck, K., 0, Richter, S.M., Ene, A.B., Roduner, E., Schmid, R.D., Urlacher, V.B. , 2008. Cloning and characterization of a new laccase from Bacillus licheniformis catalyzing dimerization of phenolic acids. Appl Microbiol Biotechnol 79: 217-224 Medline

[203] Li, Y. , 0, Dietrich, M., Schmid, R.D., He, B., Ouyang, P., Urlacher, V.B. , 2008. Identification and functional expression of a Delta9-fatty acid desaturase from Psychrobacter urativorans in Escherichia coli. Lipids 43: 207-213 Medline

Publications 2007:

[202] Pfeffer, J., 0, Freund, A., Bel-Rhlid, R., Hansen, C.E., Reuss, M., Schmid, R.D., Maurer, S.C., 2007. Highly efficient enzymatic synthesis of 2-monoacylglycerides and structured lipids and their production on a technical scale. Lipids 42: 947-953 Medline

[201] Yu, M., 0, Lange, S., Richter, S., Tan, T., Schmid, R.D., 2007. High-level expression of extracellular lipase Lip2 from Yarrowia lipolytica in Pichia pastoris and its purification and characterization. Protein Expr. Purif. 53: 255-263 Medline

[200] Soliman, N.A., 0, Knoll, M., Abdel-Fattah, Y.R., Schmid, R.D., Lange, S., 2007. Molecular cloning and characterization of thermostable esterase and lipase from Geobacillus thermoleovorans YN isolated from desert soil in Egypt. Process Biochem 42: 1090-1100

[199] Schmid, R.D., 0, Urlacher, V.B., 2007. . : 0-0

[198] Romankiewicz, A. , 0, Busch, A. , Laschat, S., Schmid, R.D., Urlacher, V.B., 2007. Separation and dentification of oxidized terpenoids by thin-layer chromatograph. Anal Letters 40(7): 1487--1495

Publications 2006:

[197] Seifert, A., 0, Tatzel, S., Schmid, R.D., Pleiss, J., 2006. Multiple molecular dynamics simulations of human P450 monooxygenase CYP2C9: the molecular basis of substrate binding and regioselectivity toward warfarin. Proteins 64: 147-155 Medline

[196] Richter, S., 0, Nieveler, J., Schulze, H., Bachmann, T.T., Schmid, R.D., 2006. High yield production of a recombinant Nippostrongylus brasiliensis acetylcholinesterase mutant in Pichia pastoris and its purification. Biotechnol Bioeng 93: 1017-1022 Medline

[195] Urlacher,V.B., 0, Makhsumkhanov, A., Schmid, R.D., 2006. Biotransformation of ionones by engineered cytochrome P450 BM-3. Appl Microbiol Biotechnol 70: 53-59 Medline

[194] Momoi, K., 0, Hofmann, U., Schmid, R.D., Urlacher, V.B., 2006. Reconstitution of beta-carotene hydroxylase activity of thermostable CYP175A1 monooxygenase. Biochem Biophys Res Commun 339: 331-336 Medline

[193] Eiben, S., 0, Kaysser, L., Maurer, S., Kuhnel, K., Urlacher, V.B., Schmid, R.D., 2006. Preparative use of isolated CYP102 monooxygenases-a critical appraisal. J Biotechnol 124: 662-669 Medline

[192] Liu, D., 0, Schmid, R.D., Rusnak, M., 2006. Functional expression of Candida antarctica lipase B in the Escherichia coli cytoplasm-a screening system for a frequently used biocatalyst. Appl Microbiol Biotechnol 72: 1024-1032 Medline

[191] Shumyantseva, V.V., 0, Bulko, T.V., Samenkova, N.F., Kuznetsova, G.P., Usanov, S.A., Schulze, H., Bachmann, T.T., Schmid, R.D., Archakov, A.I., 2006. A new format of electrodes for the electrochemical reduction of cytochromes P450. J Inorg Biochem 100: 1353-1357 Medline

[190] Pfeffer, J., 0, Richter, S., Nieveler, J., Hansen, C.E., Rhlid, R.B., Schmid, R.D., Rusnak, M., 2006. High yield expression of Lipase A from Candida antarctica in the methylotrophic yeast Pichia pastoris and its purification and characterisation. Appl Microbiol Biotechnol 72: 931-938 Medline

[189] Budde, M., 0, Morr, M., Schmid, R.D., Urlacher, V.B., 2006. Selective hydroxylation of highly branched fatty acids and their derivatives by CYP102A1 from Bacillus megaterium. Chembiochem 7: 789-794 Medline

[188] Urlacher, V.B., 0, Schmid, R.D., 2006. Recent advances in oxygenase-catalyzed biotransformations. Curr Opin Chem Biol 10: 156-161 Medline

[187] Lentz, O., 0, Feenstra, A., Habicher, T., Hauer, B., Schmid, R.D., Urlacher, V.B., 2006. Altering the regioselectivity of cytochrome P450 CYP102A3 of Bacillus subtilis by using a new versatile assay system. Chembiochem 7: 345-350 Medline

[186] Schmid, R.D., 0, 2006. Bio-Cluster und Start-ups - eine japanische Innovationsoffensive. Nachr Chem Tech Lab : 986-988

[185] Liu, L., 0, Schmid, R.D., Urlacher, V.B., 2006. Cloning, expression, and characterization of a self-sufficient cytochrome P450 monooxygenase from Rhodococcus ruber DSM 44319. Appl Microbiol Biotechnol 72: 876-882 Medline

Publications 2005:

[184] Li, Q.S., 0, Ogawa, J., Schmid, R.D., Shimizu, S., 2005. Indole hydroxylation by bacterial cytochrome P450 BM-3 and modulation of activity by cumene hydroperoxide. Biosci Biotechnol Biochem 69: 293-300 Medline

[183] Karpushova, A., 0, Brümmer, F., Barth, S., Lange, S., Schmid, R.D., 2005. Cloning, recombinant expression and biochemical characterisation of novel esterases from Bacillus sp. associated with the marine sponge Aplysina aerophoba. Appl Microbiol Biotechnol 67: 59-69 Medline

[182] Sulistyaningdyah, W.T., 0, Ogawa, J., Li, Q.S., Maeda, C., Yano, Y., Schmid, R.D., Shimizu, S., 2005. Hydroxylation activity of P450 BM-3 mutant F87V towards aromatic compounds and its application to the synthesis of hydroquinone derivatives from phenolic compounds. Appl Microbiol Biotechnol 67: 556-562 Medline

[181] Zhang, Y., 0, Muench, S.B., Schulze, H., Perz, R., Yang, B., Schmid, R.D., Bachmann, T.T., 2005. Disposable biosensor test for organophosphate and carbamate insecticides in milk. J Agric Food Chem 29: 5110-5115 Medline

[180] Schulze, H., 0, Muench, S.B., Villatte, F., Schmid, R.D., Bachmann, T.T, 2005. Highly sensitive insecticide detection through protein engineering of Nippostrongylus brasiliensis acetylcholinesterase B. Anal Chem 77: 5823-5830 Medline

[179] Villatte, F., 0, Schulze, H., Schmid, R.D., Bachmann, T.T., 2005. Use of acetylcholinesterase and its modifications for biosensoring. : 189-205 Medline

[178] Maurer, S.C., 0, Schmid, R.D., 2005. Biocatalysis for the epoxidation and hydroxylation of fatty acids and fatty alcohols. : 1-46 Medline

[177] Maurer, S., 0, Kühnel, K., Kaysser, L.O., Eiben, S., Schmid, R.D., Urlacher, V.B., 2005. Catalytic hydroxylation in biphasic systems using CYP102A1 (P450 BM-3) mutants. Adv Synth Catal 347: 1090-1098

[176] Schuster, S., 0, Enzelberger, M., Trauthwein, H., Schmid, R.D., Urlacher, V.B., 2005. pHluorin-based in vivo assay for hydrolase screening. Anal Chem 77: 2727-2732 Medline

[175] Appel, D. , 0, Schmid, R.D., Dragan, C.A., Bureik, M., Urlacher, V.B., 2005. A fluorimetric assay for cortisol. Anal Bioanal Chem 383: 182-186 Medline

[174] Rusnak, M., 0, Nieveler, J., Schmid, R.D., Petri, R., 2005. The putative lipase, AF1763, from Archaeoglobus fulgidusis a carboxylesterase with a very high pH optimum. Biotechnol Lett 11: 743-748 Medline

[173] Roh C, Villatte F, Kim BG, Schmid RD., 0, 2005. In-gel patch electrophoresis: a new method for environmental DNA purification. Electrophoresis 26: 3055-3061 Medline

[172] Schmid, R.D, 0, 2005. BioEngineering in China. Transkript 10: 81-83

[171] Dietrich, M., 0, Grundmann, L., Kurr, K., Valinotto, L., Saussele, T., Schmid, R.D., Lange, S., 2005. Recombinant production of human microsomal cytochrome P450 2D6 in the methylotrophic yeast Pichia pastoris. Chembiochem 6: 2014-2022 Medline

[170] Li, H.M., 0, Mei, L.H., Urlacher, V., Schmid, R.D., 2005. Cytochrome P450BM-3 mutants with improved catalytic properties of hydroxylating indole to indigo by error-prone PCR. Prog Biochem Biophys 32: 630-635

Publications 2004:

[169] Barth, S., 0, Fischer, M., Schmid, R.D., Pleiss, J., 2004. Sequence and structure of epoxide hydrolases: a systematic analysis. Proteins 55: 846-855 Medline

[168] Doderer, K., 0, Schmid, R.D., 2004. Fluorometric assay for determining epoxide hydrolase activity. Biotechnol Lett 26: 835-839 Medline

[167] Schulze, H., 0, Nonhoff, U., Villatte, F., Schmid, R.D., Bachmann, T.T., 2004. Highly sensitive neurotoxin detection using disposable acetylcholinesterase biosensors at elevated temperatures. Sensor Letters 2: 121-124

[166] Barth, S., 0, Fischer, M., Schmid, R.D., Pleiss, J., 2004. The database of epoxide hydrolases and haloalkane dehalogenases: one structure, many functions. Bioinformatics 20: 2845-2847 Medline

[165] Schulze, H., 0, Schmid, R.D., Bachmann, T.T., 2004. Activation of phosphorothionate pesticides based on a cytochrome P450 BM-3 (CYP102 A1) mutant for expanded neurotoxin detection in food using acetylcholinesterase biosensors. Anal Chem 76: 1720-1725 Medline

[164] Kauffmann, I.M., 0, Schmitt, J., Schmid, R.D., 2004. DNA isolation from soil samples for cloning in different hosts . Appl Microbiol Biotechnol 64: 665-670 Medline

[163] Lentz, O., 0, Urlacher, V.B., Schmid, R.D., 2004. Substrate specificity of native and mutated cytochrome P450 (CYP102A3) from Bacillus subtilis. J Biotechnol 108: 41-49 Medline

[162] Blasco, F., 0, Kauffmann, I., Schmid, R.D., 2004. CYP175A1 from Thermus thermophilus HB27, the first beta-carotene hydroxylase of the P450 superfamily . Appl Microbiol Biotechnol 64: 671-674 Medline

[161] Urlacher, V.B., 0, Lutz-Wahl, S., Schmid, R.D., 2004. Microbial P450 enzymes in biotechnology . Appl Microbiol Biotechnol 64: 317-325 Medline

[160] Urlacher, V.B., 0, Schmid, R.D., 2004. Protein engineering of the cytochrome P450 monooxygenase from Bacillus megaterium. Methods Enzymol 388: 208-224 Medline

[159] Grimm, V., 0, Ezaki, S., Susa, M., Knabbe, C., Schmid, R.D., Bachmann, T.T., 2004. Use of DNA microarrays for rapid genotyping of TEM beta-lactamases that confer resistance. J Clin Microbiol 42: 3766-3774 Medline

[158] Yu, X., 0, Susa, M., Knabbe, C., Schmid, R.D., Bachmann, T.T., 2004. Development and validation of a diagnostic DNA microarray to detect quinolone-resistant Escherichia coli among clinical isolates. J Clin Microbiol 42: 4083-4091 Medline

[157] Budde, M., 0, Maurer, S.C., Schmid, R.D., Urlacher, V.B., 2004. Cloning, expression and characterisation of CYP102A2, a self-sufficient P450 monooxygenase from Bacillus subtilis. Appl Microb Biotech 66: 180-186 Medline

[156] Sulistyaningdyah, W.T., 0, Ogawa, J., Li, Q.S., Shinkyo, R., Sakaki, T., Inouye, K., Schmid, R.D., Shimizu, S., 2004. Metabolism of polychlorinated dibenzo-p-dioxins by cytochrome P450 BM-3 and its mutant. Biotechnol Lett 26: 1857-1860 Medline

[155] Schmid, R.D., 0, Pleiss, J., Urlacher, V., 2004. Biokatalyse: Selektivoxidation von C-H-Bindungen mit O2. Nachr Chem 52: 767-772 Medline

Publications 2003:

[154] Oelschlaeger, P., 0, Lange, S., Schmitt, J., Siemann, M., Reuss, M., Schmid, R.D., 2003. Identification of factors impeding the production of a single-chain antibody fragment in Escherichia coli by comparing in vivo and in vitro expression. Appl Microbiol Biotechnol 61: 123-132 Medline

[153] Villatte, F., 0, Schulze, H., Schmid, R.D., Bachmann, T.T., 2003. A long insertion reverts the functional effect of a substitution in acetylcholinesterase. Protein Eng 16: 463-465 Medline

[152] Henke, E., 0, Bornscheuer, U.T., Schmid, R.D., Pleiss, J., 2003. A molecular mechanism of enantiorecognition of tertiary alcohols by carboxylesterases. ChemBioChem 4: 485-493 Medline

[151] Oelschlaeger, P., 0, Schmid, R.D., Pleiss, J., 2003. Insight into the mechanism of the IMP-1 metallo-beta-lactamase by molecular dynamics simulations. Protein Eng 16: 341-350 Medline

[150] Oelschlaeger, P., 0, Schmid, R.D., Pleiss, J., 2003. Modeling domino effects in enzymes: molecular basis of the substrate specificity of the bacterial metallo-beta-lactamases IMP-1 and IMP-6. Biochemistry 42: 8945-8956 Medline

[149] Yano, J.K., 0, Blasco, F., Li, H., Schmid, R.D., Henne, A., Poulos, T.L., 2003. Preliminary characterization and crystal structure of a thermostable cytochrome P450 from thermus thermophilus. J Biol Chem 278: 608-616 Medline

[148] Schulze, H., 0, Vorlova, S., Villatte, F., Bachmann, T.T, Schmid, R.D., 2003. Design of acetylcholinesterases for biosensor applications. Biosens Bioelectron 18: 201-209 Medline

[147] Schmid, R.D., 0, 2003. Pocket Guide to Biotechnology and Genetic Engineering. : 1-360

[146] Quyen, D.T., 0, Schmidt-Dannert, C., Schmid, R.D., 2003. High-level expression of a lipase from Bacillus thermocatenulatus BTL2 in Pichia pastoris and some properties of the recombinant lipase. Protein Expr Purif 28: 102-110 Medline

[145] Maurer, S., 0, Schulze, H., Schmid, R.D., Urlacher, V.B., 2003. Immobilisation of P450 BM-3 and an NADP+ cofactor recycling system: towards a technical appication of heme-containing monooxygenases i fine chemical synthesis. Adv Synth Catal 345: 802-810

[144] Schmid, R.D., 0, Ma, L., 2003. Chinese Bio Today. Chinese Bio Today.com : 0-0

[143] Schmid, R.D., 0, Lian, M., 2003. Biowissenschaften in China. Nachr Chem 51: 527-531

[142] Doderer, K., 0, Schmitt, J., Hauer, B., Schmid, R.D., 2003. High-throughput screening for bacterial epoxide hydrolyase activity. Anal Biochem 321: 131-134

[141] Bessler, C., 0, Schmitt, J., Maurer, K.H., Schmid, R.D., 2003. Directed evolution of a bacterial alpha-amylase: toward enhanced pH-performance and higher specific activity. Protein Sci 12: 2141-2149 Medline

Publications 2002:

[140] Yan, Y., 0, Bornscheuer, U.T., Schmid, R.D., 2002. Efficient water removal in lipase-catalyzed esterifications using a low-boiling-point azeotrope. Biotechnol Bioeng 78: 31-34 Medline

[139] Vorlova, S., 0, Bornscheuer, U.T., Gatfield, I., Hilmer, J.M., Bertram,H.J., Schmid, R.D., 2002. Enantioselective Hydrolysis of d,l-Menthyl Benzoate to L-(-)-Menthol by Recombinant Candida rugosa Lipase LIP1. Adv Synth Catal 344: 1152-1155

[138] Urlacher, V., 0, Schmid, R.D., 2002. Biotransformations using prokaryotic P450 monooxygenases. Curr Opin Biotechnol 13: 557-564 Medline

[137] Oelschlaeger, P., 0, Srikant-Iyer, S., Lange, S., Schmitt, J., Schmid, R.D., 2002. Fluorophor-linked immunosorbent assay: a time- and cost-saving method for the characterization of antibody fragments using a fusion protein of a single-chain antibody fragment and enhanced green fluorescent protein. Anal Biochem 309: 27-34 Medline

[136] Villatte, F., 0, Schulze, H., Schmid, R.D., Bachmann, T.T., 2002. A disposable acetylcholinesterase-based electrode biosensor to detect anatoxin-a(s) in water. Anal Bioanal Chem 372: 322-326 Medline

[135] Schulze, H., 0, Scherbaum, E, Anastassiades, M., Vorlova, S., Schmid, R.D., Bachmann, T.T., 2002. Development, validation, and application of an acetylcholinesterase-biosensor test for the direct detection of insecticide residues in infant food. Biosens Bioelectron 17: 1095-1105 Medline

[134] Schulze, H., 0, Schmid, R.D., Bachmann, T.T., 2002. Rapid detection of neurotoxic insecticides in food using disposable acetyicholinesterase-biosensors and simple solvent extraction. Anal Bioanal Chem 372: 268-272 Medline

[133] Mbeunkui, F., 0, Richaud, C., Etienne, A.L., Schmid, R.D., Bachmann, T.T, 2002. Bioavailable nitrate detection in water by an immobilized luminescent cyanobacterial reporter strain. Appl Microbiol Biotechnol 60: 306-312 Medline

[132] Kusharyoto, W., 0, Pleiss, J., Bachmann, T.T., Schmid, R.D., 2002. Mapping of a hapten-binding site: molecular modeling and site-directed mutagenesis study of an anti-atrazine antibody. Protein Eng 15: 233-241 Medline

[131] Schmitt, J., 0, Brocca, S., Schmid, R.D., Pleiss, J., 2002. Blocking the tunnel: engineering of Candida rugosa lipase mutants with short chain length specificity. Protein Eng 15: 595-601 Medline

[130] Gentner, C., 0, Schmid, R.D., Pleiss, J., 2002. Primary alcohols in a ring structure: quantifying enantioselectivity of Pseudomonas cepacia lipase by an in silico assay. Colloids Surf B Biointerfaces 26: 57-66

[129] Bathelt, C., 0, Schmid, R.D., Pleiss, J., 2002. Regioselectivity of CYP2B6: homology modeling, molecular dynamics simulation, docking. J Mol Model 8: 327-335 Medline

[128] Shumyantseva, V.V., 0, Bulko, T.V., Schmid, R.D., Archakov, A.I., 2002. Photochemical properties of a riboflavins/cytochrome P450 2B4 complex. Biosens Bioelectron 17: 233-238 Medline

[127] Shumyantseva, V.V., 0, Bulko, T.V., Usanov, S.A., Schmid, R.D., Nicolini, C., Archakov, A.I., 2002. Construction and characterization of bioelectrocatalytic sensors based on cytochromes P450. J Inorg Biochem 87: 185-190 Medline

[126] Mbeunkui, F., 0, Richaud, C., Etienne, A.L., Schmid, R.D., Bachmann, T.T., 2002. Bioavailable nitrate detection in water by an immobilized luminescent cyanobacterial reporter strain. Appl Microbiol Biotechnol 60: 306-312 Medline

Publications 2001:

[125] Villatte, F., 0, Bachmann, T.T., Hussein, A.S., Schmid, R.D., 2001. Acetylcholinesterase assay for rapid expression screening in liquid and solid media. BioTechniques 30: 81-86 Medline

[124] Minning, S., 0, Serrano, A., Ferrer, P., Sola, C., Schmid, R.D., Valero, F., 2001. Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. J Biotechnol 86: 59-70 Medline

[123] Li, Q.S., 0, Schwaneberg, U., Fischer, M., Schmitt, J., Pleiss, J., Lutz-Wahl, S., Schmid, R.D., 2001. Rational evolution of a medium chain-specific cytochrome P-450 BM-3 variant. Biochim Biophys Acta 1545: 114-121 Medline

[122] Ivanov, Y.D., 0, Kanaeva, I.P., Gnedenko, O.V., Pozdnev, V.F., Shumyantseva, V.V., Samenkova, N.F., Kznetsova, G.P., Tereza, A.M., Schmid, R.D., 2001. Optical biosensor investigation of interactions of biomembrane and water-soluble cytochromes P450 and their redox partners with covalently immobilized phosphatidylethanolamine layers. J Mol Recognit 14: 185-196 Medline

[121] Villatte, F., 0, Bachmann, T.T., Hussein, A.S., Schmid, R.D., 2001. Expression level of heterologous proteins in Pichia pastoris is influenced by flasks design. Appl Microbiol Biotechnol 55: 463-465 Medline

[120] Lange, S., 0, Schmitt, J., Schmid, R.D., 2001. High-level expression of the recombinant, atrazine-specific Fab fragment K411B by the methylotrophic yeast Pichia pastoris. J Immunol Methods 255: 103-114 Medline

[119] Lange, S., Musidlowska, A., Schmidt-Dannert, C., Schmitt, J., Bornscheuer, U. T., 0, 2001. Cloning, functional expression, and characterization of recombinant pig liver esterase. ChemBioChem 2: 576-582

[118] Schreiter, P.P., 0, Gillor, O., Post, A., Belkin, S., Schmid, R.D., Bachmann, T.T., 2001. Monitoring of phosphorus bioavailability in water by an immobilized luminescent cyanobacterial reporter strain. Biosens Bioelectron 16: 811-818 Medline

[117] Baumeister, F., 0, Konig, A., Schmid, R.D., Kretschmer, A., Bachmann, T.T., Metzger, J.W., 2001. Biosensors watching over the nitrification at wastewater treatment plants. 166: 175-185

[116] Schulz, T., 0, Schmid, R.D., Pleiss, J., 2001. Structural basis of stereoselectivity in Candida rugosa lipase-catalyzed hydrolysis of secondary alcohols. J Mol Model 7: 265-270

[115] Kahlow, U.H., 0, Schmid, R.D., Pleiss, J., 2001. A model of the pressure dependence of the enantioselectivity of Candida rugosa lipase towards (±)-menthol. Protein Sci 10: 1942-1952 Medline

[114] Traub, P.C., 0, Schmidt-Dannert, C., Schmitt, J., Schmid, R.D., 2001. Gene synthesis, expression in E. coli, and in vitro refolding of Pseudomonas sp. KWI 56 and Chromobacterium viscosum lipases and their chaperones. Appl Microbiol Biotechnol 55: 198-204 Medline

[113] Lentz, O., 0, Li, Q.S., Schwaneberg, U., Lutz-Wahl, S., Fischer, P., Schmid, R.D., 2001. Modification of the fatty acid specificity of cytochrome P450 BM-3 from Bacillus megaterium by directed evolution: a validated assay. J Mol Catal B Enzym 15: 123-133

[112] Li, Q.S., 0, Ogawa, J., Schmid, R.D., Shimizu, S., 2001. Engineering cytochrome P450 BM-3 for oxidation of polycyclic aromatic hydrocarbons. Appl Environ Microbiol 67: 5735-5739 Medline

[111] Li, Q.S., 0, Ogawa, J., Schmid, R.D., Shimizu, S., 2001. Residue size at position 87 of cytochrome P450 BM-3 determines its stereoselectivity in propylbenzene and 3-chlorostyrene oxidation. FEBS Lett 508: 249-252 Medline

[110] Man, A., 0, Liu, A.M.F., Somers, N.A., Kazlauskas, R.J., Brush, T.S., Zocher, F., Enzelberger, M.M., Bornscheuer, U.T., Horsman, G.P., 2001. Mapping the substrate selectivity of new hydrolases using colorimetric screening: lipases from Bacillus thermocatenulatus and Ophiostoma piliferum, esterases from Pseudomonas fluorescens and Streptomyces diastatochromogenes. Biochimica et Biophysica Acta-Protein Structure and molecular Enzymology; Tetrahedron Asymmetry 12: 545-556

[109] Yan, Y., 0, Bornscheuer, U.T., Stadler, G., Lutz-Wahl, S., Reuss, M., Schmid, R.D., 2001. Production of sugar fatty acid esters by enzymatic esterification in a stirred-tank membrane reactor: optimization of parameters by response surface methodology. J Am Oil Chem Soc 78: 147-153

[108] Yan, Y., 0, Bornscheuer, U.T., Stadler, G., Lutz-Wahl, S., Otto, R.T., Reuss, M., Schmid, R.D., 2001. Regioselective lipase-catalyzed synthesis of glucose ester on preparative scale. Eur J Lipid Sci Technol 103: 583-587

Publications 2000:

[107] Zocher, F., 0, Enzelberger, M.M., Bornscheuer, U.T., Hauer, B., Wohlleben, W.W., Schmid, R.D., 2000. Epoxide hydrolase activity of Streptomyces strains. J Biotechnol 77: 287-292 Medline

[106] Pleiss, J., 0, Fischer, M., Scheib, H., Schulz, T., Schmid, R.D., 2000. Modelling structure-function relationship of lipase binding sites-a case study of specificity and selectivity. : 21-39

[105] Pleiss, J., 0, Scheib, H., Schmid, R.D., 2000. The his gap motif in microbial lipases: a determinant of stereoselectivity toward triacylglycerols and analogs. Biochimie 82: 1043-1052 Medline

[104] Peters, H., 0, Schmidt-Dannert, C, Cao, L., Bornscheuer, U.T., Schmid, R.D., 2000. Purification and reconstitution of an integral membrane protein, the photoreaction center of Rhodobacter sphaeroides, using synthetic sugar esters. BioTechniques 28: 1214-1219 Medline

[103] Otto, R.T., 0, Scheib, H., Bornscheuer, U.T., Pleiss, J., Syldatk, C., Schmid, R.D., 2000. Substrate specificity of lipase B from Candida antarctica in the synthesis of arylaliphatic glycolipids. J Mol Catal B Enzym 8: 201-211

[102] Li, Q.S., 0, Schwaneberg, U., Fischer, P., Schmid, R.D., 2000. Directed evolution of the fatty-acid hydroxylase P450 BM-3 into an indole-hydroxylating catalyst. Chemistry 6: 1531-1536 Medline

[101] Köhler, S., 0, Belkin, S., Schmid, R.D., 2000. Reporter gene bioassays in environmental analysis. Fesenius J Anal Chem 366: 769-779 Medline

[100] Köhler, S., 0, Bachmann, T.T., Schmitt, J., Belkin, S., Schmid, R.D., 2000. Detection of 4-chlorobenzoate using immobilized recombinant Escherichia coli reporter strains. Sens Actuators B 70: 139-144

[99] Kovac, A., 0, Scheib, H., Pleiss, J., Schmid, R.D., Paltauf, F., 2000. Molecular basis of lipase stereoselectivity. Eur J Lipid Sci Tech 102: 61-77

[98] Enzelberger, M.M., 0, Minning, S., Schmid, R.D., 2000. Designing new metal affinity peptides by random mutagenesis of a natural metal-binding site. J Chromatogr A 898: 83-94 Medline

[97] Bornscheuer, U.T., 0, Pleiss, J., Schmidt-Dannert, C., Schmid, R.D., 2000. Lipases from Rhizopus species: genetics, structure and applications. : 115-134

[96] Bachmann, T.T., 0, Leca, B., Vilatte, F., Marty, J.L., Fournier, D., Schmid, R.D., 2000. Improved multianalyte detection of organophosphates and carbamates with disposable multielectrode biosensors using recombinant mutants of Drosophila acetylcholinesterase and artificial neural networks. Biosens Bioelectron 15: 193-201 Medline

[95] Shumyantseva, V.V., 0, Bulko, T.V., Bachmann, T.T., Bilitewski, U., Schmid, R.D., Archakov, A.I., 2000. Electrochemical reduction of flavocytochromes 2B4 and 1A2 and their catalytic activity. Arch Biochem Biophys 377: 43-48 Medline

[94] Schulz, T., 0, Pleiss, J., Schmid, R.D., 2000. Stereoselectivity of Pseudomonas cepacia lipase towards secondary alcohols: a quantitative model. Protein Sci 9: 1053-1062 Medline

[93] Pleiss, J., 0, Fischer, M., Peiker, M., Thiele, C., Schmid, R.D., 2000. Lipase Engineering Database: understanding and exploiting sequence-structure-function relationships. J Mol Catal B: Enzym 10: 491-508

[92] Hwang, B.Y., 0, Scheib, H., Pleiss, J., Kim, B.G., Schmid, R.D., 2000. Computer-aided molecular modeling of the enantioselectivity of Pseudomonas cepacia lipase toward g- and d-lactones. J Mol Catal B Enzym 10: 223-231

[91] Schwaneberg, U., 0, Appel, D., Schmitt, J., Schmid, R.D., 2000. P450 in biotechnology: zinc driven w-hydroxylation of p-nitrophenoxydodecanoic acid using P450 BM-3 F87A as a catalyst. J Biotechnol 84: 249-257 Medline

Publications 1999:

[90] Zocher, F., 0, Enzelberger, M.M., Bornscheuer, U.T., Hauer, B., Schmid, R.D., 1999. A colorimetric assay suitable for screening epoxide hydrolase activity. Anal Chim Acta 391: 345-351

[89] Yan, Y., 0, Bornscheuer, U.T., Cao, L., Schmid, R.D., 1999. Lipase-catalyzed solid-phase synthesis of sugar fatty acid esters. Removal of by-products by azeotropic distillation. Enzyme Microb Technol 25: 725-728

[88] Yan, Y., 0, Bornscheuer, U.T., Schmid, R.D., 1999. Lipase-catalyzed synthesis of vitamin c fatty acid esters. Biotechnol Lett 21: 1051-1054

[87] Shumyantseva, V.V., 0, Bulko, T.V., Alexandrova, S.A., Sokolov, N.N., Bachmann, T.T., Schmid, R.D., Archakov, A.I., 1999. N-terminal truncated cytochrome P450 2B4: catalytic activities and reduction with alternative electron sources. Biochem Biophys Res Commun 263: 678-680 Medline

[86] Schwaneberg, U., 0, Schmidt-Dannert, C., Schmitt, J., Schmid, R.D., 1999. A continuous spectrophotometric assay for P450 BM-3, a fatty acid hydroxylating enzyme, and its mutant F87A. Anal Biochem 269: 359-366 Medline

[85] Schwaneberg, U., 0, Sprauer, A., Schmidt-Dannert, C., Schmid, R.D., 1999. P450 monooxygenase in biotechnology 1. Single-step, large-scale purification method for cytochrome P450 BM-3 by anion-exchange chromatography. J Chromatogr A 848: 149-159 Medline

[84] Schmid, U., 0, Bornscheuer, U.T., Soumanou, M.M., McNeill, G.P., Schmid, R.D., 1999. Highly selective synthesis of 1,3-oleoyl-2-palmitoylglycerol by lipase catalysis. Biotechnol Bioeng 64: 678-684 Medline

[83] Scheib, H., 0, Pleiss, J., Kovac, A., Paltauf, F., Schmid, R.D., 1999. Stereoselectivity of Mucorales lipases toward triradylglycerols--a simple solution to a complex problem. Protein Sci 8: 215-221 Medline

[82] Quyen, D.T., 0, Schmidt-Dannert, C., Schmid, R.D., 1999. High-level formation of active Pseudomonas cepacia lipase after heterolgous expression of the encoding gene and its modified chaperone in Escherichia coli and rapid in vitro refolding. Appl Environ Microbiol 65: 787-794 Medline

[81] Pleiss, J., 0, Schmid, R.D., 1999. Bioinformatics and the internet. Chemistry International 21: 33-36

[80] Pleiss, J., 0, Mionetto, N., Schmid, R.D., 1999. Probing the acyl binding site of acetylcholinesterase by protein engineering. J Mol Catal B: Enzym 6: 287-296

[79] Minning, S., 0, Scheib, H., Traub, P., Schmitt. J., Schmid, R.D., 1999. A toolbox of recombinant microbial lipases. : 313-332

[78] Minning, S., 0, Weiss, A., Bornscheuer, U.T., Schmid, R.D., 1999. Determination of peracid and putative enzymatic peracid formation by an easy colorimetric assay. Anal Chim Acta 378: 293-298

[77] König, A., 0, Bachmann, T.T., Metzger, J.W., Schmid, R.D., 1999. Disposable sensor for measuring the biochemical oxygen demand for nitrification and inhibition of nitrification in wastewater. Applied Microbiology and Biotechnology 51: 112-117 Medline

[76] Ji, H.S., 0, McNiven, S., Yano, K., Ikebukuro, K., Bornscheuer, U.T., Schmid, R.D., Karube, I., 1999. Highly sensitive trilayer piezoelectric odor sensor. Anal Chim Acta 387: 39-45

[75] Cao, L., 0, Bornscheuer, U.T., Schmid, R.D., 1999. Lipase-catalyzed solid-phase synthesis of sugar esters. Influence of immobilization on productivity and stability of the enzyme. J Mol Catal B Enzym 6: 279-285

[74] Bachmann, T.T., 0, Schmid, R.D., 1999. A disposable multielectrode biosensor for the discrimination of organophosphate and carbamate insecticides in the submicromolar range using native and recombinant variants of acetylcholinesterase. Anal Chim Acta 401: 95-103

[73] Cuong, N.V., 0, Bachmann, T.T., Schmid, R.D., 1999. Development of a dipstick immunoassay for quantitative determination of 2,4-dichlorophenoxyacetic acid in water, fruit and urine samples. Fresenius J Anal Chem 364: 584-589

[72] Hauer, B., 0, Schmid, R.D., Enzelberger, M., Minning, S., 1999. Peptides for creation of fusion proteins and their purification by metal ion affinity chromatography. WO 9960134: 0-0

[71] Soumanou, M.M., 0, Bornscheuer, U.T., Schmid, U., Schmid, R.D., 1999. Crucial role of support and water activity on the lipase-catalyzed synthesis of structured triglycerides. Biocatal Biotransform 16: 443-459

Publications 1998:

[70] Yano, K., 0, Karube, I., Bornscheuer, U.T., Schmid, R.D., 1998. Development of an odorant sensor using polymer-coated quartz crystals modified with unusual lipids. Biosens Bioelectron 13: 397-405

[69] Wagegg, T., 0, Enzelberger, M.M., Bornscheuer, U.T., Schmid, R.D., 1998. The use of methoxy acetoxy esters significantly enhances reaction rates in the lipase-catalyzed preparation of enantiopure 1-(4-chloro phenyl) ethyl amines. J Biotechnol 61: 75-78

[68] Takahashi, S., 0, Ueda, M., Atomi, H., Beer, H.D., Bornscheuer, U.T., Schmid, R.D., Tanaka, A., 1998. Extracellular production of active Rhizopus oryzae lipase by Saccharomyces cerevisiae. J Ferment Bioeng 86: 164-168

[67] Soumanou, M.M., 0, Schmid, U., Bornscheuer, U.T., Schmid, R.D., 1998. Lipase-katalysierte Synthese von strukturierten Triglyceriden. Schriftenreihe 10: 184-190

[66] Soumanou, M.M., 0, Bornscheuer, U.T., Schmid, R.D., 1998. Two-step enzymatic reaction for the synthesis of pure structured triacylglycerides. J Am Oil Chem Soc 75: 703-710

[65] Soumanou, M.M., 0, Bornscheuer, U.T., Schmid, U., Schmid, R.D., 1998. Synthesis of structured triglycerides by lipase catalysis. Fett/Lipid 100: 156-160

[64] Schmid, U., 0, Bornscheuer, U.T., Soumanou, M.M., McNeill, G,P,, Schmid, R.D., 1998. Optimization of the reaction conditions in the lipase-catalyzed synthesis of structured triglycerides. J Am Oil Chem Soc 75: 1527-1531

[63] Schmid, R.D., 0, Verger, R., 1998. Lipases: interfacial enzymes with attractive applications. Angew Chem Int Ed 37: 1608-1633

[62] Scheib, H., 0, Pleiss, J., Stadler, P., Kovac, A., Potthoff, A., Haalck, L., Spener, F., Paltauf, F., Schmid, R.D., 1998. Rational design of Rhizopus oryzae lipase with modified stereoselectivity toward triradylglycerols. Protein Eng 11: 675-682 Medline

[61] Rubtsova, M.Y., 0, Wittmann, C., Egorov, A.M., Schmid, R.D., 1998. Chemiluminescent immunoassay: application of a portable scanning luminometer for the determination of 2,4-dichlorophenoxyacetic acid in microtiter and membrane strip format. Food Agric Immunol 9: 235-247

[60] Rubtsova, M.Y., 0, Egorov, A.M., Schmid, R.D., 1998. Simultaneous determination of several pesticides with chemiluminescent immunoassay on a multi-spot membrane strip. Food Agric Immunol 10: 223-235

[59] Pleiss, J., 0, Fischer, M., Schmid, R.D., 1998. Anatomy of lipase binding sites: the scissile fatty acid binding site. Chem Phys Lipids 93: 67-80 Medline

[58] Otto, R.T., 0, Bornscheuer, U.T., Scheib, H., Pleiss, J., Syldatk, C., Schmid, R.D., 1998. Lipase-catalyzed esterification of unusual substrates: synthesis of glucuronic acid and ascorbic acid. Biotechnol Lett 20: 1091-1094

[57] Otto, R.T., 0, Bornscheuer, U.T., Syldatk, C., Schmid, R.D., 1998. Synthesis of aromatic n-alkyl-glucoside esters in a coupled beta-glucosidase and lipase reaction. Biotechnology Letters 20: 437-440

[56] Otto, R.T., 0, Cao, L., Bornscheuer, U.T., Syldatk, C., Schmid, R.D., 1998. Lipase-catalyzed solid phase synthesis of 6-O-monoesters from beta-D(+)-glucose and aromatic acids. Synthesis : 0-0

[55] Mosiello, L., 0, Segre, L., Chiavarini, S., Spano, M., Cremisini, C., Kimmel, T., Bäumner, A.J., Schmid, R.D., 1998. Dipstick immunoassay format for atrazine and terbuthylazine analysis in water samples. J Agric Food Chem 46: 3847-3851

[54] Minning, S., 0, Schmidt-Dannert, C., Schmid, R.D., 1998. Functional expression of Rhizopus oryzae lipase in Pichia pastoris: high-level production and some properties. J Biotechnol 66: 147-156 Medline

[53] Lutz-Wahl, S., 0, Fischer, P., Schmidt-Dannert, C., Wohlleben, W., Schmid, R.D., 1998. Stereo- and regioselective hydroxylation of alpha-ionone by Streptomyces strains. Appl Environ Microbiol 64: 3878-3881 Medline

[52] Heim, J., 0, Schmidt-Dannert, C., Atomi, H., Schmid, R.D., 1998. Functional expression of a mammalian acetylcholinesterase in Pichia pastoris: comparison to acetylcholinesterase, expressed and reconstituted from Escherichia coli. Biochim Biophys Acta 1396: 306-319 Medline

[51] Enzelberger, M.M., 0, Zocher, F., Bornscheuer, U., Schmidt-Dannert, C., Hauer, B., Schmid, R.D., 1998. Singularisation of enzyme mutants by use of a cell sorter and the green fluorescent protein. Bioforum 21: 192-194

[50] Cao, L., 0, Bornscheuer, U.T., Schmid, R.D., 1998. Lipase-catalyzed solid-phase synthesis of sugar esters, IV: Selectivity of lipases towards primary and secondary hydroxyl groups in carbohydrates. Biocatal Biotransform 16: 249-257

[49] Brocca, S., 0, Schmidt-Dannert, C., Lotti, M., Alberghina, L., Schmid, R.D., 1998. Design, total synthesis, and functional overexpression of the Candida rugosa lip1 gene coding for a major industrial lipase. Protein Sci 7: 1415-1422 Medline

[48] Beimborn, D., 0, Flemming, H.C., Grummt, T., Hansen, P.D., Hock, B., Kanne, R., Kohler, H.P., Ludwig, W., Obst, U., 1998. Bioeffects-related environmental analytics. Part 1. Limits of chemical and biological analytics. Labor-Fachzeitschrift 42: 905-908

[47] Beer, H.D., 0, McCarthy, J.E., Bornscheuer, U.T., Schmid, R.D., 1998. Cloning, expression, characterization and role of the leader sequence of a lipase from Rhizopus oryzae. Biochim Biophys Acta 1399: 173-180 Medline

[46] Bäumner, A.J., 0, Schmid, R.D., 1998. Development of a new immunosensor for pesticide detection: a disposable system with lipsome-enhancement and amperometric detection. Biosens Bioelectron 13: 519-529 Medline

[45] Bachmann, T.T., 0, Bilitewski, U., Schmid, R.D., 1998. A microbial sensor based on Pseudomonas putida for phenol, benzoic acid and their monochlorinated derivatives which can be used in water and n-hexane. Anal Lett 31: 2361-2373

[44] Kisselev, P., 0, Wessel, R., Pisch, S., Bornscheuer, U., Schmid, R.D., Schwarz, D., 1998. Branched phosphatidylcholines stimulate activity of cytochrome P450SCC (CYP11A1) in phospholipid vesicles by enhancing cholesterol binding, membrane incorporation and protein exchange. J Biol Chem 273: 1380-1386 Medline

[43] Rua, M.L., 0, Atomi, H., Schmidt-Dannert, C., Schmid, R.D., 1998. High-level expression of the thermoalkalophilic lipase from Bacillus thermocatenulatus in Escherichia coli. Appl Microbiol Biotechnol 49: 405-410 Medline

[42] Beyersdorf-Radeck, B. , 0, Riedel, K., Karlson, U., Bachmann, T.T., Schmid, R.D., 1998. Screening of xenobiotic compounds degrading microorganisms using biosensor techniques. Microbiol Res 153: 239-245 Medline

[41] Schmidt-Dannert, C., 0, Pleiss, J., Schmid, R.D., 1998. A toolbox of recombinant lipases for industrial applications. Ann N Y Acad Sci 864: 14-22 Medline

Publications 1997:

[40] Yano, K., 0, Yoshitake, H., Bornscheuer, U.T., Schmid, R.D., Ikebukuro, K., Yokoyama, K., Masuda, Y., Karube, I., 1997. Development of a chemical vapor sensor using piezoelectric quartz crystals with coated unusual lipids. Anal Chim Acta 340: 41-48

[39] Wittmann, C., 0, Schmid, R.D., Löffler, S., Zell, A., 1997. Application of a neural network for pattern recognition of pesticides in water samples by different immunochemical techniques. : 343-360

[38] Wittmann, C., 0, Riedel, K., Schmid, R.D., 1997. Microbial and enzyme sensors for environmental monitoring. : 299-332

[37] Wittmann, C., 0, Schmid, R.D., 1997. Bioaffinity sensors for environmental monitoring. : 333-347

[36] Soumanou, M.M., 0, Bornscheuer, U.T., Menge, U., Schmid, R.D., 1997. Synthesis of structured triglycerides from peanut oil with immobilized lipase. J Am Oil Chem Soc 74: 427-433

[35] Schwarz, D., 0, Kisselev, P., Pfeil, W., Pisch, S., Bornscheuer, U., Schmid, R.D., 1997. Evidence that nonbilayer phase propensity of the membrane is important for the side chain cleavage activity of cytochrome P450SCC. Biochemistry 36: 14262-14270 Medline

[34] Schwarz, D., 0, Kisselev, P., Wessel, R., Pisch, S., Bornscheuer, U., Schmid, R.D., 1997. Possible involvement of nonbilayer lipids in the stimulation of the activity of cytochrome P450SCC (CAP11A1) and its propensity to induce vesicle aggregation. Chem Phys Lipids 85: 91-99 Medline

[33] Schrag, J.D., 0, Li, Y., Cygler, M., Lang, D., Burgdorf, T., Hecht, H.J., Schmid, R., Schomburg, D., Rydel, T., 1997. The open conformation of a Pseudomonas lipase. Structure 5: 187-202 Medline

[32] Schmidt-Dannert, C., 0, Rua, M.L., Schmid, R.D., 1997. Two novel lipases from the thermophile Bacillus thermocatenulatus: cloning, overexpression in E. coli, purification and properties. Methods Enzymol 284: 194-220 Medline

[31] Schmidt-Dannert, C., 0, Rua, M.L., Wahl, S., Schmid, R.D., 1997. Bacillus thermocatenulatus lipase: a thermoalkalophilic lipase with interesting properties. Biochem Soc Trans 25: 178-182 Medline

[30] Rua, M.L., 0, Schmidt-Dannert, C., Wahl, S., Sprauer, A., Schmid, R.D., 1997. Thermoalkalophilic lipase of Bacillus thermocatenulatus large-scale production, purification and properties: aggregation behaviour and its effect on activity. J Biotechnol 56: 89-102 Medline

[29] Pisch, S., 0, Bornscheuer, U.T., Meyer, M.M., Schmid, R.D., 1997. Properties of unusual phospholipids .4. Chemoenzymatic synthesis of phospholipids bearing acetylenic fatty acids. Tetrahedron 53: 14627-14634

[28] Peters, H., 0, Schmidt-Dannert, C., Schmid, R.D., 1997. The photoreaction center of Rhodobacter sphaeroides. Mater Sci Eng C 4: 227-232

[27] Nishihara, H., 0, Okamura, T., Schmid, R.D., Hauck, A., Reuss, M., 1997. Biotechnological potential of P450 monooxygenases. High-level production of bovine cytochrome P450c17 monooxygenase during medium cell density culture of a recombinant yeast, Saccharomyces cerevisiae GRF 18 (YEp-Toku1). J Biotechnol 56: 57-61 Medline

[26] Mionetto, A., 0, Morel, N., Massoulié, J., Schmid, R.D., 1997. Biochemical determination of insecticides via cholinesterases. 1. Acetylcholinesterase from rat brain: functional expression using a baculovirus system, and biochemical characterization. Biotechnol Tech 11: 805-812

[25] Kalisz, H.M., 0, Hendle, J, Schmid, R.D., 1997. Structural and biochemical properties of glycosylated and deglycosylated glucose oxidase from Penicillium amagasakiense. Appl Microbiol Biotechnol 47: 502-507 Medline

[24] Holzwarth, H.C., 0, Pleiss, J., Schmid, R.D., 1997. Computer-aided modelling of stereoselective triglyceride hydrolysis catalyzed by Rhizopus oryzae lipase. J Mol Catal B Enzym 3: 73-82

[23] Haalck, L., 0, Paltauf, F., Pleiss, J., Schmid, R.D., Spener, F., Stadler, P., 1997. Stereoselectivity of lipase from Rhizopus oryzae toward triacylglycerols and anlogs: computer aided modelling and experimental validation. Methods Enzymol 284: 353-376

[22] Cao, L., 0, Fischer, A., Bornscheuer, U.T., Schmid, R.D., 1997. Lipase-catalyzed solid phase synthesis of sugar fatty acid esters. Biocatalysis and Biotransformations 14: 269-283

[21] Pleiss, J., 0, Mionetto, N., Schmid, R.D., 1997. Protein engineering of rat brain acetylcholinesterase: a point mutation enhances sensitivityto pesticides. Protein Eng 10: 66-66

[20] Enzelberger, M.M., 0, Bornscheuer, U.T., Gatfield, I., Schmid, R.D., 1997. Lipase-catalysed resolution of gamma- and delta-lactones. J Biotechnol 56: 129-133 Medline

[19] Catoni, E., 0, Schmidt-Dannert, C., Brocca, S., Schmid, R.D., 1997. Overexpression of lipase A and B of Geotrichum candidum in Pichia pastoris: high-level production and some properties of functional expressed lipase B. Biotechnol Tech 11: 689-695

Publications 1996:

[18] Schwarz, D., 0, Kisselev, P., Wessel, R., Jüptner, O., Schmid, R.D., 1996. Alpha-branched 1,2-diacyl phosphatidylcholines as effectors of activity of cytochrome P450SCC (CYP11A1). Modeling the structure of the fatty acyl chain region of cardiolipin.. Journal of Biological Chemistry 271: 12840-12846 Medline

[17] Wittmann, C., 0, Bilitewski, U., Giersch, T., Kettling, U., Schmid, R.D., 1996. Development and evaluation of a dipstick immunoassay format for the determination of atrazine residues on-site. Analyst 121: 863-869 Medline

[16] Smidt, H., 0, Fischer, A., Fischer, P., Schmid, R.D., 1996. Preparation of optically pure chiral amines by lipase-catalyzed enantioselective hydrolysis of N-acyl-amines. Biotechnol Tech 10: 335-338

[15] Schmidt-Dannert, C., 0, Rua, M.L., Atomi, H., Schmid, R.D., 1996. Thermoalkalophilic lipase of Bacillus thermocatenulatus. I. molecular cloning, nucleotide sequence, purification and some properties. BBA-Lipid Lipid Met 1301: 105-114 Medline

[14] Lang, D., 0, Hofmann, B., Haalck, L., Hecht, H.J., Spener, F., Schmid, R.D., Schomburg, D., 1996. Crystal structure of a bacterial lipase from Chromobacterium viscosum ATCC 6918 refined at 1.6 angstroms resolution. J Mol Biol 259: 704-717 Medline

[13] Heidt, M., 0, Bornscheuer, U.T., Schmid, R.D., 1996. Studies on the enantioselectivity in the lipase-catalyzed synthesis of monoacylglycerols from isopropylidene glycerol. Biotechnol Tech 10: 25-30

[12] Gaziola, L., 0, Bornscheuer, U.T., Schmid, R.D., 1996. A rapid and effective separation of enantiomers of glycerol derivatives by gas chromatography and their lipase-catalyzed biotransformation. Enantiomer 1: 49-54

[11] Drees, R.C., 0, Pleiss, J., Schmid, R.D., 1996. Highly immersive molecular modeling (HIMM): an architecture for the integration of molecular modelling and virtual reality. : 190-192

[10] Cao, L., 0, Bornscheuer, U.T., Schmid, R.D., 1996. Lipase-catalyzed solid-phase synthesis of sugar esters. Lipid/Fett 98: 332-335

[9] Bornscheuer, U., 0, Gaziola, L., Schmid, R.D., 1996. Application of vinyl esters for the lipase-catalyzed high-yield synthesis of monoacylglycerols. Ann NY Acad Sci 799: 757-761

[8] Bäumner, A.J., 0, Kummer, T., Schmid, R.D., 1996. Liposome-based immunosensors. 1. Influence of hapten spacer length on liposome binding efficiency. Anal Lett 29: 2601-2613

[7] Beer, H.D., 0, Wohlfahrt, G., Schmid, R.D., McCarthy, J.E.G., 1996. The folding and activity of the extracellular lipase of Rhizopus oryzae are modulated by a prosequence. Biochem J 319: 351-359 Medline

[6] Beer, H.D., 0, Wohlfahrt, G., McCarthy, J.E.G., Schomburg, D., Schmid, R.D., 1996. Analysis of the catalytic mechanism of a fungal lipase using computer-aided design and structural mutants. Protein Eng 9: 507-517 Medline

Publications 1995:

[5] Schmid, R.D., 0, Menge, U., Schomburg, D., Spener, F., 1995. Towards novel biocatalysts via protein design: the case of lipases. FEMS Microbiol Rev 16: 253-253

[4] Schmid, R.D., 0, 1995. In Vorbereitung auf große Wachstumsmärkte: Biotechnologie und Gentechnik in Japan, Korea, China und Taiwan. Pharmazeutische Industrie 57: 370-379

[3] Schmid, R.D., 0, Chung, B.H., Jones, A.J., Saono, S., Scriven, J., Tsai, J.H.J., 1995. . 12: 371-432

Publications 1994:

[2] Rurup, J., 0, Lauer, F., Drewes, A., Wray, V., Schmid, R.D., 1994. Properties of unusual phospholipids, II: Synthesis, NMR studies and monolayer investigations of diacylglycerophosphocholines containing alpha-branched acyl chains. Chem Phys Lipids 72: 175-183 Medline

[1] Rurup, J., 0, Mannova, M., Brezesinki, G., Schmid, R.D., 1994. Properties of unusual phospholipids: I. Synthesis, monolayer investigations and calorimetry of diacylglycerophosphocholines containing monoacetylenic acyl chains. Chemistry and Physics of Lipids 70: 187-198 Medline

Monographs, chapters in monographs:

[12] Schmid, R.D., 0, Chung, B.H., Jones, A.J., Saono, S., Scriven, J., Tsai, J.H.J., 1995. . In: 0. (eds. Biotechnology: a Multi-Volume Comprehensive Treatise. Modern Biotechnology: Legal, Economic and Ethical Dimensions. ). Rehm, H.J.; Reed, G.; Bauer, D. Wiley-VCH.

[11] Drees, R.C., 0, Pleiss, J., Schmid, R.D., 1996. Highly immersive molecular modeling (HIMM): an architecture for the integration of molecular modelling and virtual reality. In: 0. (eds. Computer science and biology : proceedings of the German Conference on Bioinformatics (GCB 96)). Hofestädt, R.; Löffler, M.; Schomburg, D. Universität Leipzig.

[10] Wittmann, C., 0, Schmid, R.D., Löffler, S., Zell, A., 1997. Application of a neural network for pattern recognition of pesticides in water samples by different immunochemical techniques. In: 0. (eds. ACS Symposium Series No. 657, Immunochemical Technology for Environmental Applications). Aga, D.S.; Thurman, E.M. Oxford University Press.

[9] Wittmann, C., 0, Riedel, K., Schmid, R.D., 1997. Microbial and enzyme sensors for environmental monitoring. In: 0. (eds. Handbook of Biosensors and Electronic Noses: Medicine, Food and the Environment). Kress-Rogers, E. CRC Press.

[8] Wittmann, C., 0, Schmid, R.D., 1997. Bioaffinity sensors for environmental monitoring. In: 0. (eds. Handbook of Biosensors and Electronic Noses: Medicine, Food and the Environment). Kress-Rogers, E. CRC Press.

[7] Minning, S., 0, Scheib, H., Traub, P., Schmitt. J., Schmid, R.D., 1999. A toolbox of recombinant microbial lipases. In: 0. (eds. Lipases and Lipids Structure, function and biotechnological applications Anwendungen ). Kokotos, G. Crete University Press.

[6] Pleiss, J., 0, Fischer, M., Scheib, H., Schulz, T., Schmid, R.D., 2000. Modelling structure-function relationship of lipase binding sites-a case study of specificity and selectivity. In: 0. (eds. Lipases and Lipids Structure, function and biotechnological applications ). Kokotos, G. Crete University Press.

[5] Bornscheuer, U.T., 0, Pleiss, J., Schmidt-Dannert, C., Schmid, R.D., 2000. Lipases from Rhizopus species: genetics, structure and applications. In: 0. (eds. Protein engineering in industrial biotechnology). Alberghina, L. CRC Press .

[4] Baumeister, F., 0, Konig, A., Schmid, R.D., Kretschmer, A., Bachmann, T.T., Metzger, J.W., 2001. Biosensors watching over the nitrification at wastewater treatment plants. In: 0. (eds. Stuttgarter Berichte zur Siedlungswasserwirtschaft[Stickstoff im Wasser/Abwasser]). Kommissionsverlag R. Oldenbourg.

[3] Schmid, R.D., 0, 2003. Pocket Guide to Biotechnology and Genetic Engineering. In: 0. (eds. Pocket Guide to Biotechnology and Genetic Engineering). Schmid, R.D. Wiley VCH.

[2] Villatte, F., 0, Schulze, H., Schmid, R.D., Bachmann, T.T., 2005. Use of acetylcholinesterase and its modifications for biosensoring. In: 0. (eds. Recent Trends in the Acetylcholinesterase System). Parveen, M.; Kumar, S. IOS Press.

[1] Maurer, S.C., 0, Schmid, R.D., 2005. Biocatalysis for the epoxidation and hydroxylation of fatty acids and fatty alcohols. In: 0. (eds. Handbook of industrial biocatalysis, 2005). Hou, C.T. CRC Press LLC.

Patent applications

[26] Jan Weile, Milorad Susa, Cornelius Knabbe, Rolf Schmid, Till Bachmann, "Detecting antibiotic resistance- and virulence-associated mutations in Pseudomonas aeruginosa using microarry-assisted genotyping", Eppendorf AG, Germany, 2006

[25] Verena Grimm, Milorad Susa, Cornelius Knabbe, Rolf Schmid, Till Bachmann, "Detecting antibiotic resistance associated with SNP8s) in SHV-type ß-lactamase (bla-SHV) gene using microarray-assisted genotyping", Eppendorf AG, Germany, 2006

[24] Verena Grimm,Satoshi Eszaki, Milorad Susa, Cornelius Knabbe, Rolf Schmid, Till Bachmann, "DNA microarray for detecting ß-lactam resistant microorganism in a biol. sample and SNP in -lactamase gene", Eppendorf AG, Germany, 2005

[23] Cornelius Bessler, Susanne Schmitz, Jutta Schmitt, Rolf Schmid, ”Substitution variants of α-amylase with improved activity in alkaline conditions for use in cleaning compostions”, Henkel KGaA, Germany, 2004

[22] Markus Matuscheck, Bernhard Hauer, Rolf Schmid, Isabelle Kaufmann, Francesca Blasco, Claudia Schmidt-Dannert, ”Production of hydroxycarotenes by Escherichia coli expressing a thermostable cytochrome P 450 monooxygenase from Thermus thermophilus”, BASF AG, Germany, 2004

[21] Bernhard Hauer, Markus Matuschek, Rolf Schmid, Christiane Buta, Isabelle Kauffmann, Katrin Laemmle, Hubert Zipper, "Method for producing normalized gene libraries of soil microorganisms and theier use in screening for novel enzymes", BASF AG, Germany, 2003

[20] Alexander Makower, Jan Halamek, Petr. Skladal, Holger Schulze, Rolf D. Schmid, Till Bachmann, Dorothea Pfeiffer, Verfahren und Assay zum Nachweis und zur Bestimmung der Konzentration von Enzymhemmstoffen und/oder deren inaktiven Vorstufen, BST BioSensor Technologie GmbH, Berlin, 2002.

[19] Uwe Bornscheuer, Rolf D. Schmid, Sandra Vorlova, Ian Gatfield, Jens-Michael Hilmer, "Verfahren zur Gewinnung von L-Menthol", Haarmann & Reimer GmbH, Germany, 2001.

[18] Bernhard Hauer, Ulrich Schwaneberg, Sabine Lutz-Wahl, Daniel Appel, Rolf Schmid, Qing-shan Li, "Neue Cytochrom P450-Monooxygenasen und deren Verwendung zur Oxidation von organischen Verbindungen", BASF AG, Germany, 2000.

[17] Stefania Brocca, Uwe Bornscheuer, Jürgen Pleiss, Rolf D. Schmid, Ulrike Schmid, Jutta Schmitt, "Modified lipolytic enzymes and their use", EPO 002000513.0, Unilever N. V., 2000.

[16] Uwe Bornscheuer, Rolf D. Schmid, Christoph Syldatk, Youchun Yan, Ralf Otto, "Verfahren zur selektiven Veresterung von Polyolen",DE 199 24 221 A1, Henkel KGaA, 2000.

[15] Francesca Blasco, Jutta Schmitt, Rolf D. Schmid, Bernhard Hauer (Göttingen), "Cytochrom P450 Monooxygenasen aus thermophilen Bakterien", O.Z. 0050/51821, BASF AG, 2000.

[14] Youchun Yan, Ralf T. Otto, Uwe T. Bornscheuer, Christoph Syldatk and Rolf D. Schmid, "Procedure for selective esterifications of polyols" (B) DE 19920558.2 (1999)

[13] Q.-S. Li, U. Schwaneberg, M. Fischer, J. Pleiss, R. D. Schmid "Neue Cytochrom P450-Monooxygenasen und deren Verwendung zur Oxidation N-heterocyclischer Aromaten". BASF AG Ludwigshafen, DE (1999)

[12] F. Zocher, M. Enzelberger, U. Bornscheuer, W. Wohlleben, B. Hauer, R. D. Schmid "Epoxidhydrolasen aus Streptomyces". BASF AG Ludwigshafen, DE 199 35 113.9 (1999)

[11] U. Schwaneberg, D. Appel, B. Hauer, R. D. Schmid. "Elektronendonorsystem für Enzyme". BASF AG Ludwigshafen, DE 199 35 115.5 (1999)

[10] R. Otto, C. Syldatk, L. Cao, U. Bornscheuer, R. D. Schmid. "Verfahren zur selektiven Veresterung von Polyolen. German Patent DE 197 53 789 A1 (1999)

[9] M. Enzelberger, S. Minning, R. D. Schmid, B. Hauer, "Neue Peptidfragmente zur Reinigung von Proteinen". BASF AG, Ludwigshafen, DE

[8] S. Minning, U. Bornscheuer, R. D. Schmid, "Azo dye for detection of percarboxylic acids" DE 19651797 A1 980618 ( Chem. Abstr. 129:68839), Germany1998.

[7] S. Brocca, C. Schmidt-Dannert, M. Lotti, L. Alberghina, R. D. Schmid, "Total synthesis and functional overexpression of a Candida rugosa lLIP1 gene coding for a major industrial ipase", PCT/NL97/00524, Unilever, Netherlands 1997.

[6] Sabine Wahl, Claudia Schmidt-Dannert, Rolf D. Schmid, Wolfgang Wohlleben, Oliver Scherr, Peter Fischer, "Verfahren zur biotechnologischen Oxidation von substituierten Trimethylcyclohexenylverbindungen", BASF AG, Germany, 1998.

[5] Ralf Otto, Holger Scheib, Christoph Syldatk, Uwe Bornscheuer, Rolf D. Schmid, "Verfahren zur Herstellung von Uronsäureestern", DE 198 39 219.2, Henkel KGaA,1998.

[4] Uwe T. Bornscheuer, Mohamed M. Soumanou, Rolf D. Schmid, Ulrike Schmid, "Preparation of symmetrical triglycerides ABA", EP 882792, (Chem. Abstr. 130:51410),Unilever N. V., 1998.

[3] Ralf T. Otto, Uwe T. Bornscheuer, Christoph Syldatk, Rolf D. Schmid, "Verfahren zur selektiven Veresterung von Polyolen", DE 197 53 789.8, Henkel KGaA, 1997.

[2] H. Smidt, A. Fischer, P. Fischer, R. D. Schmid, U. Stelzer, "Production of optically active amines",Germany Offen.; DE 19507217 A1, Bayer AG, Germany 1996.

[1] Rolf D. Schmid, Stefan Minning, Uwe Bornscheuer, "Azofarbstoff zur Detektion von Percarbonsäuren", DE 19651797, Henkel KGaA, Germany 1996.